Guanidiinitiosyanaatti molekyylibiologiaan

Toimittaja: Thermo Scientific

Varoitus

J65104.36EA 591 EUR
J65104.36 J65104.30 J65104.18
Guanidiinitiosyanaatti molekyylibiologiaan
Guanidiinitiosyanaatti

Guanidine thiocyanate is used for RNA isolation and can solubilise proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.


Soluble in H₂O (1420 g/L) at 20°C, and ethanol (5%).


Varoitus: Light Sensitive. Keep container tightly closed and store away from oxidising agents.

Kaava: CH₅N₃·CHNS
MW: 118,16 g/mol
Sulamispiste: 118…122 °C
Tiheys: 1,29
Storage Temperature: Huoneenlämpö
MDL numero: MFCD00013027
CAS-numero: 593-84-0
EINECS: 209-812-1

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Appearance (Colour) White to off-white
Form Crystalline powder
Assay (Titration) 99.0% min.
DNase None detected
Protease activity None detected
RNase None detected
UV/Vis A280 (70%, water) 0.8
UV/Vis A300 (6M, water) 0.1
UV/Vis A410 (6M, water) 0.1

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