Ideal for tagged biomolecule purification using IMAC.
- Metal-chelate affinity chromatography exploits the affinity of proteins or other molecules for metal ions
- Can be used in combination with standard chromatography columns, disposable gravity flow columns, disposable Nanosep® centrifugal filters and AcroPrep™ multi-well filter plates
- Uses tridentate IDA (iminodiacetic acid) as a chelating ligand agent. This ligand is immobilised on the HyperCel™ base sorbent, a stable, robust and well-known sorbent used for both research and industrial-scale protein separations
- Prior to use, metal ions can be immobilised on the IMAC HyperCel™ resin
Average particle size: 90 µm
Typical working pressure: <1 bar (100 kPa, 14 psi)
Capacity for metal ions: 40 - 70 µmol Cu² per ml of sorbent
Ionic capacity: 90 - 140 µeq/ml
Regeneration: 0,5 - 1,0 M NaOH
Pressure resistance: <3 bar (300 kPa, 44 psi)
Working pH: 3 - 12
Storage temperature: 2 - 8 °C
