Affinity chromatography column, HiPrep™ Heparin FFToimittaja: Cytiva
28-9365-49EA 719 EUR28-9365-49
Affinity chromatography column, HiPrep™ Heparin FF
HiPrep™ Heparin FF 16/10 is prepacked with Heparin Sepharose™ 6 fast flow for preparative purification of proteins with affinity for heparin. Heparin is a mucopolysaccharide consisting of a repeating of α-L-dipyranuronicdimer acid 2-sulphate, and 2-deoxy-2-sulfamino-α-D-glucopyranose 6-sulphate, with a molecular weight range of 5000 to 30000.
- 20 ml column optimised for convenient scale-up purification
- Immobilised heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors
- Simple operation with single pump configurations, as well as ÄKTA design systems
- Excellent flow characteristics
Heparin functions as a high-capacity cation exchanger, due to its anionic sulphate groups. Gradient elution with salt is most commonly used. Heparin is coupled to Sepharose™ 6 Fast Flow with a chemically optimized linkage.