Phenol : chloroform : iso-amyl alcohol (25:24:1) (v/v/v) ≥99%, Biotechnology Grade, low pH

Toimittaja: VWR Chemicals

0966-400MLEA 344 EUR
0966-400ML 0966-100ML
Phenol : chloroform : iso-amyl alcohol (25:24:1) (v/v/v) ≥99%, Biotechnology Grade, low pH
Phenol : Chloroform : iso-Amyl alcohol

Phenol / Chloroform #2- A 25:24:1 mixture of phenol, chloroform, and isoamyl alcohol useful for preparing RNA with maximal recovery of poly (A+) RNA for subsequent mRNA purification and generation of cDNA.


  • Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
  • Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
  • Optimized pH for the Purification of RNA
  • Long Term Stability > One Year


For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

MDL numero: MFCD00133763
CAS-numero: 136112-00-0
UN: 2810
ADR: 6.1,II

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Testitulosten erittely

O.D.@ 330 nm ≤0.2
O.D.@ 405 nm ≤0.1
O.D.@ 510 nm ≤0.1
pH @ 25 °C 4.9 – 5.5
Purity ≥99 %

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