Protein solutions as dilute as 1 ng/ml, if treated with Perfect-FOCUS™, can be quantitatively precipitated into a small volume. Protein precipitation is not affected by the presence of detergents or other common laboratory agents.
The collected protein sample has a conductivity of approximately 40 to 50 µS and is substantially free from non-protein agents, making the protein sample ideal for critical IEF and 2D gel electrophoresis studies, with improved spot resolution and greatly reduced spot streaking. Perfect-FOCUS™ treated protein samples are compatible with mass spectrometry analysis and show identical peptide spectra.