Ready to screen human cancer cell line blots.
These premade blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using Western transfer buffer. The blots are then ready to be blocked and probed with the antibodies of choice.
Features
Blot lane order:
Protein marker
Acute promyelocytic leukemia (HL-60)
T lymphocyte lymphoma (HUT-78)
B cell lymphoma (BJAB)
Mammary adenocarcinoma (MCF7)
Amelanotic melanoma (C32)
Acute T cell leukemia (JURKAT)
Chronic Myelogenous leukemia (K562)
Epidermoid carcinoma (A-431)
Epithlioid carcinoma (HeLa)
Burkitt's lymphoma (Ramos)
Prostate adenocarcinoma (LNCap)
Hepatoblastoma (HepG2)
Neuroepithelioma (SK-N-MC)
