Anti-Connexin-40 Sheep Polyclonal Antibody

Toimittaja: Biosensis
BSENS-063-100EA 312 EUR
Anti-Connexin-40 Sheep Polyclonal Antibody
FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in lung. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily.

Immunohistochemistry: Antibody detects Cxn 40 in rat tissues and arterial endothelial cells. The authors report that the density of Cx40 plaques was significantly greater in the caudal artery (CA) than in the thoracic aorta (ThA), whereas no such difference was seen for Cx37 and Cx43. Expression of Cx40 was absent from the media of both thoracic and caudal artery tissues (see Rummery, NM et al 2002 for more staining specifics). Refer to Rummery et al 2002 for IHC method.
Western Blot: Antibody is not recommended for western blots by Biosensis, however, it does react in westerns with Cxn 40 specific material. The authors report that the antibody develops numerous bands in westerns blots, only some of which are removed upon peptide treatment (see Rummery, NM et al 2002). The Cx40/254 antibody specifically recognized a band of 40 kDa from lung, caudal artery (CA), and thoracic aorta (ThA) but not liver (online Figure VIIA, +/- peptide). In the lung, however, a band at 45 kDa also appeared to be reduced with Cxn 40 peptide addition.Refer to Rummery et al 2002 for WB method.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: Connexin-40
Clonality: Polyclonal
Conjugation: Unconjugated
Epitope: rat Connexin-40 protein (aa: 253-269)
Host: Sheep
Isotype: IgG
Reactivity: Rat
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