Anti-glutathione peroxidase 1 Sheep Polyclonal Antibody

Toimittaja: Biosensis
BSENS-072-100EA 345 EUR
Anti-glutathione peroxidase 1 Sheep Polyclonal Antibody
Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. Two alternatively spliced isoforms have been identified.

This antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1) protein.
Application Information:
IHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000 depending on tissue and detection method. For western blotting a dilution range of 1: 10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 is recommended. This antiserum has extremely high titre, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: glutathione peroxidase 1
Clonality: Polyclonal
Conjugation: Unconjugated
Epitope: amino acids 175-194 of Human lutathione peroxidase 1 ( GPx-1)
Host: Sheep
Isotype: IgG
Reactivity: Rat, Human
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