Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner. This antibody is raised in sheep to detect the prodomain of BDNF and not the mature peptide.
Confirmed to react with purified human proBDNF, crossreact with mouse and rat proBDNF
ELISA, Western Blot, immunocytochemistry, biological neutralization of proBDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Epitope: Multiple epitopes because of the nature of the immunogen
Reactivity: Human, Mouse, Rat