Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Specifically recognizes the light neurofilament subunit NF-L (~70 kDa) in WB.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Neurofilament Light
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody
Host: Rabbit
Isotype: IgG
Reactivity: Human, Rat, Mouse, Cat, Chicken
![Left: Mouse cerebellum section stained with rabbit antibody to NF-L (red) and MBP (green) by IHC. Right: Western blot analysis of tissue lysates for NF-L expression. [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord.](https://fi.vwr.com/stibo/low_res/std.lang.all/70/81/26507081.jpg "Left: Mouse cerebellum section stained with rabbit antibody to NF-L (red) and MBP (green) by IHC. Right: Western blot analysis of tissue lysates for NF-L expression. [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord.")
![Left: Mouse cerebellum section stained with rabbit antibody to NF-L (red) and MBP (green) by IHC. Right: Western blot analysis of tissue lysates for NF-L expression. [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord.](https://fi.vwr.com/stibo/grid/std.lang.all/70/81/26507081.jpg)
