This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Ubiquitin C Terminal Hydrolase 1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody
Host: Rabbit
Isotype: IgG
Reactivity: Human, Rat, Mouse, Bovine, Pig
