Anti-Tyrosine Hydroxylase Rabbit Polyclonal Antibody

Toimittaja: Biosensis
BSENR-118-100EA 334 EUR
BSENR-118-100
Anti-Tyrosine Hydroxylase Rabbit Polyclonal Antibody
Vasta-aineet
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.

IHC on brain shows a pattern of staining specific for TH containing neurons.
Application Information:
IHC. A dilution of 1:2000 to 1: 5000 is recommended for this application. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. Western blotting: A dilution of 1:100 - 1:500 is recommended.Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: Tyrosine Hydroxylase
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Human TH 63-78
Host: Rabbit
Isotype: IgG
Reactivity: Rat, Mouse, Guinea Pig
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