Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240kDa. However the real molecular weight is considerably less than this, at 177kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.
This antibody is specific for the 240 kDa Nestin protein by WB on developing rat brain (P18) homogenate. A much weaker band at approx. 90 kDa may also be seen. This is suggested to be a breakdown product of the 240 kDa band.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). Suggested dilution for WB is 1:1,000-5,000 and 1:250-500 for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Nestin
Clonality: Monoclonal
Clone: 4D11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG
Reactivity: Human, Rodent
![Left: Detection of nestin expression (red) in cortical neuron-glial cells from E20 rat by ICC. Cells were co-stained with chicken antibody to MAP2 (green). Blue: DAPI nuclear dye. Right: Western blot analysis of tissue and cell lysates. [1] MWM, [2] embryonic E18 rat brain, [3] C6, and [4] SH-SY5Y.](https://fi.vwr.com/stibo/low_res/std.lang.all/70/52/26507052.jpg "Left: Detection of nestin expression (red) in cortical neuron-glial cells from E20 rat by ICC. Cells were co-stained with chicken antibody to MAP2 (green). Blue: DAPI nuclear dye. Right: Western blot analysis of tissue and cell lysates. [1] MWM, [2] embryonic E18 rat brain, [3] C6, and [4] SH-SY5Y.")
![Left: Detection of nestin expression (red) in cortical neuron-glial cells from E20 rat by ICC. Cells were co-stained with chicken antibody to MAP2 (green). Blue: DAPI nuclear dye. Right: Western blot analysis of tissue and cell lysates. [1] MWM, [2] embryonic E18 rat brain, [3] C6, and [4] SH-SY5Y.](https://fi.vwr.com/stibo/grid/std.lang.all/70/52/26507052.jpg)
