Cytochrome P450 46A1 belongs to the cytochrome P450 family which are a group of monooxygenases. Cytochrome P450 46A1 is involved in the turnover of cholesterol converting it into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. This protein is expressed in the brain and mRNA has been found to be broadly distributed with higher levels in the gray matter zones and lower levels in white matter areas. It is not detected in fetal brain but its expression increases linearly with age. It localises to the endoplasmic reticulum membrane and the microsome membrane. The unprocessed precursor is 500 amino acids long and has a predicted molecular weight of 56.8KDa.
The specificity of this antibody has been confirmed by WB and ELISA against the antigen.
Application Information:
This antibody is recommended for WB and direct and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: cytochrome P450 46A1
Clonality: Monoclonal
Clone: 1A11
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity:
![Western blot detection of partial recombinant human cytochrome P450 46A1 protein with GST tag, using mouse monoclonal antibody to human cytochrome P450 46A1 [1A11], catalogue number BSENM-821-100. Note the GST tag alone has a molecµlar weight of 26KDa.](https://fi.vwr.com/stibo/low_res/std.lang.all/32/90/23163290.jpg "Western blot detection of partial recombinant human cytochrome P450 46A1 protein with GST tag, using mouse monoclonal antibody to human cytochrome P450 46A1 [1A11], catalogue number BSENM-821-100. Note the GST tag alone has a molecµlar weight of 26KDa.")
![Sandwhich ELISA using mouse monoclonal antibody to cytochrome P450 46A1 [1A11], catalogue number BSENM-821-100, to capture recombinant GST tagged cytochrome P450 46A1. The detection limit for this antibody is 0.03ng/ml.](https://fi.vwr.com/stibo/low_res/std.lang.all/29/29/23162929.jpg "Sandwhich ELISA using mouse monoclonal antibody to cytochrome P450 46A1 [1A11], catalogue number BSENM-821-100, to capture recombinant GST tagged cytochrome P450 46A1. The detection limit for this antibody is 0.03ng/ml.")
![Western blot detection of partial recombinant human cytochrome P450 46A1 protein with GST tag, using mouse monoclonal antibody to human cytochrome P450 46A1 [1A11], catalogue number BSENM-821-100. Note the GST tag alone has a molecµlar weight of 26KDa.](https://fi.vwr.com/stibo/grid/std.lang.all/32/90/23163290.jpg)
![Sandwhich ELISA using mouse monoclonal antibody to cytochrome P450 46A1 [1A11], catalogue number BSENM-821-100, to capture recombinant GST tagged cytochrome P450 46A1. The detection limit for this antibody is 0.03ng/ml.](https://fi.vwr.com/stibo/grid/std.lang.all/29/29/23162929.jpg)
