CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional regulation, hormone production, translational regulation, regulation of actin filament organisation and neurite outgrowth. CaM kinase I is involved in calcium-dependent activation of the ERK pathway. It phosphorylates proteins with the substrate consensus sequence [MVLIF}-x-R-x(2)-[ST]-x(3)-[MVLIF]. It is activated by calcium ions or calmodulin. It reaches its maximum catalytic activity when phosphorylated by CAMKK1 or CAMKK2. CaM kinase I has a length of 370 amino acids (unprocessed precursor) and a predicted molecular weight of 41.3KDa. It is a monomer that exists in many tissues and is located within the cytoplasm or nucleus. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the CaMK subfamily.
The specificity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged CaM Kinase 1 protein.
Application Information:
This antibody is recommended for WB and both direct and sandwich ELISA. This antibody has a detection limit of 0.3ng/ml for recombinant GST tagged CaM kinase 1 when used in sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: CaM kinase 1
Clonality: Monoclonal
Clone: 3G1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity:
![Sandwhich ELISA using mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number BSENM-810-100. This antibody has a detection limit of 0.3ng/ml for recombinant GST tagged CaM kinase 1 protein.](https://fi.vwr.com/stibo/low_res/std.lang.all/29/09/23162909.jpg "Sandwhich ELISA using mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number BSENM-810-100. This antibody has a detection limit of 0.3ng/ml for recombinant GST tagged CaM kinase 1 protein.")
![Mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number BSENM-810-100, detects a band of molecular weight 42KDa in human promyelocytic leukemia (HL-60) cell lysate under denaturing conditions for WB.](https://fi.vwr.com/stibo/low_res/std.lang.all/32/87/23163287.jpg "Mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number BSENM-810-100, detects a band of molecular weight 42KDa in human promyelocytic leukemia (HL-60) cell lysate under denaturing conditions for WB.")
![Sandwhich ELISA using mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number BSENM-810-100. This antibody has a detection limit of 0.3ng/ml for recombinant GST tagged CaM kinase 1 protein.](https://fi.vwr.com/stibo/grid/std.lang.all/29/09/23162909.jpg)
![Mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number BSENM-810-100, detects a band of molecular weight 42KDa in human promyelocytic leukemia (HL-60) cell lysate under denaturing conditions for WB.](https://fi.vwr.com/stibo/grid/std.lang.all/32/87/23163287.jpg)
