C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
The antibody detects mouse C-reactive protein in western blots by virtue of its epitope being the C-terminal peptide of the protein, amino acids 210-225. Because of homology, reactivity with C-terminal protein from other species is expected. The antibody also reacts in ELISA with mouse C-reactive protein
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Antigen: C-reactive Protein terminal peptide
Epitope: C-terminal region of Mouse CRP, amino acids 210-225.