This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord, brain, SHSY-5Y or HEK293 cell extract.
Application Information:
Western Blotting (WB), Immunocytochemistry (IC) and Immunohistochemistry (IH). A dilution of 1:20,000 is recommended for WB. A dilution of 1:500 - 1:2,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Ubiquitin C Terminal Hydrolase 1
Clonality: Monoclonal
Clone: BH7
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Rat, Bovine
![An image showing a section of rat spinal cord stained with Mouse monoclonal antibody to Ubiquitin C Terminal Hydrolase 1 [BH7] BSENM-1407-100 (red) and Rabbit polyclonal antibody to neurofilament H BSENR-1388-50 (green).](https://fi.vwr.com/stibo/low_res/std.lang.all/30/01/23163001.jpg "An image showing a section of rat spinal cord stained with Mouse monoclonal antibody to Ubiquitin C Terminal Hydrolase 1 [BH7] BSENM-1407-100 (red) and Rabbit polyclonal antibody to neurofilament H BSENR-1388-50 (green).")
![An image showing a section of rat spinal cord stained with Mouse monoclonal antibody to Ubiquitin C Terminal Hydrolase 1 [BH7] BSENM-1407-100 (red) and Rabbit polyclonal antibody to neurofilament H BSENR-1388-50 (green).](https://fi.vwr.com/stibo/grid/std.lang.all/30/01/23163001.jpg)
