Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.
- Contains four Fc-binding domains from Protein A and two from Protein G
- Provides binding for all antibody species and subclasses recognised by either Protein A or Protein G
Gene fusion of the Fc-binding domains of Protein A and Protein G has resulted in production of a structurally and functionally chimeric protein with broader binding than either Protein A or Protein G alone. During fusion, the Protein G gene sequence coding for the serum albumin-binding site is eliminated. This protein is expressed in E. coli and secreted into the surrounding medium during fermentation. The product obtained is consistent in quality and yield because the bacterial host is engineered to be deficient in major proteolytic activities.
