The Pierce™ Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups.
The Sulfhydryl Addition Kit uses SATA (N-succinimidyl S-acetylthioacetate) as a means of adding sulfhydryls to molecules. SATA contains an N-hydroxysuccinimide (NHS) ester that forms a stable amide bond with primary amines. Once the covalent linkage is formed, the modified molecule can be stored and the sulfhydryl group de-protected when needed. The reaction conditions for SATA are mild and non-denaturing, which preserves protein activity.
Toimitustiedot: The kit includes SATA (2 mg); hydroxylamine-HCl (5 mg); 10X conjugation buffer stock (20 ml); phosphate buffered saline pack (1 pack); dimethylformamide (DMF, 1 ml); dextran desalting column (1×5 ml); column extender (1); Ellman’s reagent (DTNB, 2 mg); and cysteine-HCl H₂O (20 mg).
