Betaine enhancer solution 5 M (5X) for PCR

Toimittaja: VWR Chemicals

Synonyms: TMG

733-2451EA 3690 EUR
733-2451 733-2450 733-1361
Betaine enhancer solution 5 M (5X) for PCR

Betaine Enhancer -liuos on erityisen tehokas paljon GC:tä sisältävien alueiden PCR-monistuksessa tai templaateissa, joissa on runsaasti sekundaarirakenteita. Sillä on alukkeen sulamislämpötilaa alentava vaikutus.

  • Enhances amplification on GC-rich and difficult DNA targets
  • Lowers the melting temperature

The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.

Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.

Varoitus: For Research Use Only. Not for use in diagnostic procedures.

Kaava: C₅H₁₁NO₂
MW: 117,15 g/mol
Storage Temperature: Pakastin
MDL numero: MFCD00012123
CAS-numero: 107-43-7

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Testitulosten erittely

Functional test - 1x Betaine Enchancer Solution is tested for amplification of 613bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions Passes test
Volume check- fill volume >= 0.1ml per tube Passes test

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