Betaine enhancer solution 5 M (5X) for PCR
Toimittaja: VWR Chemicals
Synonyms:
TMG
Betaine Enhancer -liuos on erityisen tehokas paljon GC:tä sisältävien alueiden PCR-monistuksessa tai templaateissa, joissa on runsaasti sekundaarirakenteita. Sillä on alukkeen sulamislämpötilaa alentava vaikutus.
- Enhances amplification on GC-rich and difficult DNA targets
- Lowers the melting temperature
The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.
Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.
Varoitus: For Research Use Only. Not for use in diagnostic procedures.
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Kaava:
C₅H₁₁NO₂ MW: 117,15 g/mol Storage Temperature: Pakastin |
MDL numero:
MFCD00012123 CAS-numero: 107-43-7 |
Testitulosten erittely
| Functional test - 1x Betaine Enchancer Solution is tested for amplification of 613bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions | Passes test |
| Volume check- fill volume >= 0.1ml per tube | Passes test |
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