T4 polynucleotide kinase

Toimittaja: Thermo Fisher Scientific
EK0032EA 213 EUR
EK0032 EK0031
T4 polynucleotide kinase
Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

  • Detection of DNA modification by the [³²P]-postlabelling assay
  • 5'-phosphorylation of oligonucleotides, PCR products, and DNA or RNA prior to ligation
  • Removal of 3'-phosphoryl groups
  • Phosphorylation of PCR primers

T4 polynucleotide kinase exhibits 5'-phosphatase activity and catalyses the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction). The enzyme is also a 3'-phosphatase.

Labelling 5'-termini of nucleic acids to be used as probes for hybridisation, probes for transcript mapping, markers for gel electrophoresis, primers for DNA sequencing, primers for PCR.

Varoitus: For research use only. Not for use in diagnostic procedures.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Lue lisää About VWR