Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism.
This antibody reacts with rat only. Does not react with mouse or human NGFR
Application Information:
IF: live or lightly fixed cells or tissues (acetone or 4% PFA): 2-5 ug/mL. Not suitable for western blots; not suitable for IH on formalin fixed tissues. FACS (20 ug/mL) is recommended, unfixed cells
Type: Primary
Antigen: NGFR
Clonality: Monoclonal
Clone: MC192
Conjugation: ATTO 488
Epitope: Extracellular domain of rat NGFR.
Host: Mouse
Isotype: IgG1
Reactivity: Rat
