α-Naphthyl Acetate (Non-Specific Esterase) Kit Kit formulated with all liquid reagents., Sigma-Aldrich®
Toimittaja: SIGMA-ALDRICH MICROSCOPY
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Vaara
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M-Clarity™ quality level = MQ500
Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates.
- Suitable for haematology
- Suitable for histology
According to the methodology, a-naphthyl acetate is enzymatically hydrolysed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black coloured deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes.
Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.
Kit component also available separately
α-Naphthyl acetate solution 10 mL (916)
Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mL (915)
Fast Blue BB Base solution 10 mL (917)
Sodium nitrite solution 10 mL (914)
Kit component only
Hematoxylin solution, Gill No. 3 50 mL
TRIZMAL™ 7.6 Concentrate 50 mL
Leimahduspiste:
36 °C Storage Temperature: Jääkaappi |
MDL numero:
MFCD08705438 UN: 3316 ADR: 9,II |
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