bisBenzimide H 33258 trihydrochloride pentahydrate (Hoechst 33258)

Toimittaja: Biotium

Varoitus

40045.EA 281 EUR
40045.
bisBenzimide H 33258 trihydrochloride pentahydrate (Hoechst 33258)
bisBenzimide H 33258 trihydrochloride pentahydrate (Hoechst 33258)

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Biotium also offers Hoechst 33258, 10 mg/ml in dH2O (#40044). Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.


  • λEx/λEm (with DNA) = 352/461 nm
  • Pale yellow solid soluble in water up to 2% (w/v)
  • Store at -20°C and protect from light


Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.

Kaava: C₂₅H₂₄N₆O·3HCl·5H₂O
MW: 623,96 g/mol
Storage Temperature: Pakastin
MDL numero: MFCD00012679
CAS-numero: 23491-45-4
EINECS: 245-690-6

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