illustra™ plasmidPrep Mini Spin KitToimittaja: Cytiva
28-9042-70EA 364 EUR28-9042-70 28-9042-71 28-9042-69
illustra™ plasmidPrep Mini Spin Kit
Nukleiinihapporeagenssit Nucleic Acid Purification Kits and Reagents
The illustra™ plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Produces high quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.
- Total preparation time of less than 10 minutes
- Sample size 1,5 - 3,0 ml
- Increased ease of use provided by minimal changes in pipetting volume from one part of the protocol to the next, fewer centrifugation steps and colour-coded kit components
- No organic solvents needed
The typical plasmid DNA yield from a fresh 1,5 ml culture of E. coli containing a high copy number plasmid is approximately 9 μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.
Toimitustiedot: Kits include spin columns pre-packed with a silica membrane, suspension, lysis, and neutralisation buffers, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.