EDTA buffer, pH 8.5, 10×, antigen retriever, Sigma-Aldrich®Toimittaja: Merck
E1161-100MLEA 32.4 EURE1161-100ML
EDTA buffer, pH 8.5, 10×, antigen retriever, Sigma-Aldrich®
Biokemialliset puskuriliuokset Antibody Purification Buffers
Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.
In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of EDTA buffer, pH 8.5, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens. EDTA buffer has been used to heat tissue sections in order to visualize antigens by immunohistochemical analysis.
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