SURE competent cellsToimittaja: Agilent Technologies
STRA200152EA 465 EURHEWL200152 STRA200152 STRA200227 AGLS200238 STRA200238 AGLS200152 AGLS200227
SURE competent cells
SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.
- Lack components of the pathways that catalyse the rearrangement and deletion of nonstandard secondary and tertiary structures
- Allows for blue-white colour screening
Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems.
SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryotic cells. SURE cells are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) endonuclease (endA) deficient, and recombination (recB recJ) deficient.
Varoitus: For research use only. Not for use in diagnostic procedures.