Pfu DNA ligase

Toimittaja: Agilent Technologies
AGLS600191EA 544 EUR
Pfu DNA ligase
Nukleiinihapporeagenssit Cloning Kits and Ligation
Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.

  • Highly thermostable, with half-life of over 60 minutes at 95 °C
  • Increases reliability of LCR by permitting higher melt temperatures
  • Eliminates LCR template-predenaturation step, less enzyme required for LCR

It has higher ligation specificity and lower background than Tth DNA ligase.

Toimitustiedot: Kit contains: 400 U Pfu DNA Ligase 10X reaction buffer

Varoitus: For research use only. Not for use in diagnostic procedures.
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