JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.
- Rapid, simple measurement of mitochondrial membrane potential/cell viability
- Healthy cells stain red & green, dead cells stain green
- For fluorescence microscopy, flow cytometry, or fluorescence microplate reader
- Colors Green/Red
JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells, a key indicator of cell viability.
The loss of mitochondrial membrane potential is a hallmark for apoptosis. It is an early event preceding phosphatidylserine externalization and coinciding with caspase activation. In non-apoptotic cells, JC-1 accumulates as aggregates in the mitochondrial membranes, resulting in red fluorescence. The brightness of red fluorescence is proportional to the potential and varies among different cell types. However, in apoptotic and necrotic cells, which have diminished mitochondrial membrane potential, JC-1 exists in the green fluorescent monomeric form. This kit provides a step-by-step protocol and ready-to-use reagents for performing assays for flow cytometry, fluorescence microscopy, or fluorescence microplate reader.
Spectral properties:
Ex/Em (cytoplasm): 510/527 nm (green)
Ex/Em (polarized mitochondria): 585/590 nm (red)
Kit components:
JC-1, 100X in DMSO
10X Assay Buffer
Varoitus: Store at 4 °C
