Transformation with blue and green fluorescent proteinsToimittaja: Edvotek
EDVO222EA 197 EUREDVO222
Transformation with blue and green fluorescent proteins
Opetusmateriaalit Biology Educational Materials Microbiology Learning Activities
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
- For 10 lab groups
- Complete in 50 minutes and grow overnight
Also required: 37 °C incubator, water bath, longwave UV lamp.
Toimitustiedot: Kit includes instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile pipettes, and loops.