ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.
- For rapid purification of labeled DNA (20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μl using spin-column chromatography
- Prepacked with Sephadex G-50 DNA Grade (see illustra Sephadex G-50 and G-100 DNA Grade) and pre-equilibrated in STE containing 0.15% Kathon CG/ICP Biocide
- Requires less than 4 minutes from sample application to collection of purified product
- Designed for use in a microcentrifuge
- Tested in nickase, single and double-stranded exonuclease and RNase assays
In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 to 50 μl.
