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  4. illustra™ TempliPhi™100/500 Amplification Kits, Cytiva
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illustra™ TempliPhi™100/500 Amplification Kits, Cytiva

Toimittaja: Cytiva
illustra TemliPhi 100500 DNA amplification kit
TempliPhi 100/500 amplification kits
TempliPhi 100/500 amplification kits
DNA amplification kit
TempliPhi 100/500 amplification kits
TempliPhi 100/500 amplification Kit
Rolling circle amplification process
Template preparation process
Designer protein workflow
Designer protein workflow
Transfection efficiency with supercoiled plasmid vs RCA derived DNA
Rolling circle amplification process
Template preparation process
Template prepared using TempliPhi Sequence Resolver
Designer protein workflow
Electron micrograph of nondenatured TempliPhi amplified product
illustra TemliPhi 100500 DNA amplification kit
TempliPhi 100/500 amplification kits
TempliPhi 100/500 amplification kits
DNA amplification kit
TempliPhi 100/500 amplification kits
TempliPhi 100/500 amplification Kit
Rolling circle amplification process
Template preparation process
Designer protein workflow
Designer protein workflow
Transfection efficiency with supercoiled plasmid vs RCA derived DNA
Rolling circle amplification process
Template preparation process
Template prepared using TempliPhi Sequence Resolver
Designer protein workflow
Electron micrograph of nondenatured TempliPhi amplified product
25-6400-10EA 299 EUR
25-6400-10 25-6400-50
illustra™ TempliPhi™100/500 Amplification Kits, Cytiva
Nukleiinihapporeagenssit Isothermal Amplification Reagents
TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

  • Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 hours
  • Generate microgram quantities of template DNA from picogram amounts of starting material
  • Use amplified DNA directly for cycle sequencing without purification
  • Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA
  • Simple protocol reduces time, labour, and consumables needed for template preparation, and workflow enables easy automation
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