TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.
- Ideal for DNA recovery and in-gel manipulations
- Low ionic strength
- Better resolution of large DNA fragments (>12 kb DNA)
- Convenient packaging options minimise weighing and mixing
Composition of (1X) TAE buffer: 0,04 M Tris-acetate; 0,001 M EDTA; final pH 8,0.