Exonuclease I

Toimittaja: Cytiva
E70073ZEA 368 EUR
E70073Z E70073X
Exonuclease I
  • 3'→5' single-stranded exonuclease
  • Inactivated by heating at 80°C for 15 minutes
  • Active in PCR buffers
  • Easily soluble in cold water and hot water

Digestion of single-stranded DNA by Exonuclease I releases deoxyribonucleoside 5'-mono-phosphates leaving 5'-terminal dinucleotides intact. DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups are not removed. Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli. One unit of the enzyme catalyzes the release of 10 nmol of acid soluble nucleotides in 30 minutes at 37°C.

Free of contaminating endonucleases, double-stranded exonucleases and ribonucleases. Greater than 95% pure as determined by SDS-PAGE.

illustra Exonuclease I, used together with illustra Alkaline Phosphatase for inactivation of dNTPs, provides effective cleanup of PCR reactions for subsequent use in DNA sequencing.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Lue lisää About VWR